Capacidad antagónica de cepas endófitas Trichoderma spp. frente a Lasiodiplodia theobromae in vitro, asociada a muerte regresiva en Palto (Persea americana)
| dc.contributor.advisor | León Ttacca, , Betsabé | |
| dc.contributor.author | Espinoza Huayhua, , Renzo José | |
| dc.date.accessioned | 2025-06-13T21:29:39Z | |
| dc.date.available | 2025-06-13T21:29:39Z | |
| dc.date.issued | 2025-06-13 | |
| dc.description.abstract | Lasiodiplodia theobromae es un patógeno muy importante en varias especies frutales principalmente en el cultivo de palto, causando pérdidas en la producción. A causa de ello los agricultores son dependientes del uso de productos químicos, los cuales generan contaminación al ambiente y problemas de salud en el ser humano. El objetivo fue evaluar la capacidad antagónica in vitro de cepas endófitas de Trichoderma spp. frente a Lasiodiplodia theobromae asociada a la muerte regresiva del palto (Persea americana). Se utilizó el Diseño Completamente al Azar (DCA) con 20 tratamientos + 1 testigo y 3 repeticiones, las variables evaluadas fueron: competencia, micoparasitismo y antibiosis frente a Lasiodiplodia theobromae. Los resultados mostraron que las cepas TC20 y TC12 (Trichoderma sp.) con valores 86.66% y 86.18% presentaron la mayor tasa de inhibición micelial, seguido de la TC4 (Trichoderma asperellum) con 79.81%; para la variable micoparasitismo, la TC12 y TC20 (Trichoderma sp), TC17 (Trichoderma harzianum), TC4 (Trichoderma asperellum), TC6 (Trichoderma vermificola) y TC7 (Trichoderma harzianum) mostraron un 100% de micoparatismo en comparación a las otras cepas en estudio. Finalmente, para la variable antibiosis las cepas TC12 y TC18 (Trichoderma sp.) con 77.8% y 74.1% presentaron un mayor porcentaje de antibiosis frente a Lasiodiplodia theobromae seguido de TC20 (Trichoderma sp) con 66.7%. En conclusión, las cepas endófitas extraídas de tejidos vegetales tuvieron buenos resultados en cuanto a competencia por espacio y nutrientes, micoparasitismo y antibiosis frente a Lasiodiplodia theobromae por lo que se recomienda realizar más estudios acerca de los componentes que presentan las cepas endófitas de Trichoderma. | |
| dc.description.abstract | Lasiodiplodia theobromae is a significant pathogen in various fruit species, particularly avocado in cultivation, causing production losses. As a result, farmers rely heavily on chemical products, which lead to environmental contamination and health issues in humans. The objective of this study was to evaluate the in vitro antagonistic capacity of endophytic Trichoderma spp. Strains against Lasiodiplodia theobromae associated with avocado (Persea americana) dieback. A Completely Randomized Design (CRD) was used, with 20 treatments + 1 control and 3 replications. The evaluated variables were antibiosis, mycoparasitism, and competition against Lasiodiplodia theobromae. The results showed that strains TC20 and TC12 (Trichoderma sp.) had the highest mycelial inhibition rates, with values of 86.66% and 86.18%, respectively, followed by TC4 (Trichoderma asperellum) with 79.81%. For the mycoparasitism variable, strains TC12 and TC20 (Trichoderma sp.), TC17 (Trichoderma harzianum), TC4 (Trichoderma asperellum), TC6 (Trichoderma vermificola), and TC7 (Trichoderma harzianum) exhibited 100% mycoparasitism compared to the other strains in the study. Finally, for the antibiosis variable, strains TC12 and TC18 (Trichoderma sp.) showed the highest antibiosis percentages against Lasiodiplodia theobromae, with 77.8% and 74.1%, respectively, followed by TC20 (Trichoderma sp.) with 66.7%. In conclusion, the endophytic strains extracted from plant tissues showed promising results in terms of competition for space and nutrients, mycoparasitism, and antibiosis against Lasiodiplodia theobromae. Therefore, further studies are recommended to analyze the components present in endophytic Trichoderma strains. | |
| dc.format | application/pdf | |
| dc.identifier.citation | Espinoza Huayhua, Renzo José , “Capacidad antagónica de cepas endófitas Trichoderma spp. frente a Lasiodiplodia theobromae in vitro, asociada a muerte regresiva en Palto (Persea americana).” , Tesis para optar el grado de Ingeniero Agronomo, Escuela de pregrado. Facultad de Ciencias Agrarias. Universidad Nacional de Cañete. Cañete - Perú | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14559/291 | |
| dc.language.iso | spa | |
| dc.publisher | Universidad Nacional de Cañete | |
| dc.publisher.country | PE | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Antibiosis | |
| dc.subject | competencia | |
| dc.subject | control biológico | |
| dc.subject | hongos fitopatógenos | |
| dc.subject | micoparasitismo. | |
| dc.subject.ocde | https://purl.org/pe-repo/ocde/ford#4.01.06 | |
| dc.title | Capacidad antagónica de cepas endófitas Trichoderma spp. frente a Lasiodiplodia theobromae in vitro, asociada a muerte regresiva en Palto (Persea americana) | |
| dc.type | info:eu-repo/semantics/bachelorThesis | |
| renati.advisor.dni | 41288646 | |
| renati.advisor.orcid | https://orcid.org//0000 -00024343-2431 | |
| renati.author.dni | 70299730 | |
| renati.discipline | 811036 | |
| renati.juror | Álvarez Bernaola, , Luis Armando | |
| renati.juror | Ñique Álvarez, , Manuel Alfredo | |
| renati.juror | Coaquira Incacari,, Roberto | |
| renati.level | https://purl.org/pe-repo/renati/level#tituloProfesional | |
| renati.type | https://purl.org/pe-repo/renati/type#tesis | |
| thesis.degree.discipline | Agronomía | |
| thesis.degree.grantor | Universidad Nacional de Cañete. Facultad de Ciencias Agrarias | |
| thesis.degree.name | Ingeniero Agrónomo |